Rock Band 3:n biisilista koki ennenaikaisen julkistuksen kun pelin viisut selattiin alusta loppuun Harmonixin videohaastattelun taustalla. Video ladattiin YouTubeen ja HD-kuvanlaadun ansiosta tarkkasilmäiset pystyivät hahmottamaan mitä lokakuussa soitellaan.
Harmonix suhtautui asiaan kevyesti vahvistamalla Rock Band 3:n sisällön omaan tyyliinsä:
Kaiken kaikkiaan pelilevyltä löytyy siis seuraavat musiikkiraidat:
· Amy Winehouse Rehab
· Anthrax Caught in a Mosh
· At the Drive-In One Armed Scissor
· Avenged Sevenfold Beast and the Harlot
· The B-52s Rock Lobster
· The Beach Boys Good Vibrations (Live)
· Big Country In a Big Country
· Blondie Heart of Glass
· Bob Marley & The Wailers Get Up, Stand Up
· The Bronx False Alarm
· Chicago 25 or 6 to 4
· The Cure Just Like Heaven
· David Bowie Space Oddity
· Deep Purple Smoke on the Water
· Def Leppard Foolin´
· Devo Whip It
· Dio Rainbow in the Dark
· Dire Straits Walk of Life
· The Doobie Brothers China Grove
· The Doors Break on Through (To the Other Side)
· Dover King George
· Echo & the Bunnymen The Killing Moon
· Elton John Saturday Night´s Alright For Fighting
· Faith No More MidLife Crisis
· Filter Hey Man, Nice Shot
· The Flaming Lips Yoshimi Battles the Pink Robots Pt. 1
· Foreigner Cold as Ice
· Golden Earring Radar Love
· HIM Killing Loneliness
· Huey Lewis and the News The Power of Love
· Hypernova Viva la Resistance
· Ida Maria Oh My God
· INXS Need You Tonight
· The J. Geils Band Centerfold
· James Brown I Got You (I Feel Good)
· Jane´s Addiction Been Caught Stealing
· The Jimi Hendrix Experience Crosstown Traffic
· Joan Jett I Love Rock N´ Roll
· John Lennon Imagine
· Juanes Me Enamora
· Lynyrd Skynyrd Free Bird
· Maná Oye Mi Amor
· Marilyn Manson The Beautiful People
· Metric Combat Baby
· The Muffs Outer Space
· Night Ranger Sister Christian
· Ozzy Osbourne Crazy Train
· Paramore Misery Business
· Phish Llama
· Phoenix Lasso
· The Police Don´t Stand So Close to Me
· Poni Hoax Antibodies
· Pretty Girls Make Graves Something Bigger, Something Brighter
· Primus Jerry Was a Race Car Driver
· Queen Bohemian Rhapsody
· Queens of the Stone Age No One Knows
· Rammstein Du Hast
· The Ramones I Wanna Be Sedated
· Raveonettes Last Dance
· Rilo Kiley Portions of Foxes
· Riverboat Gamblers Don´t Bury Me
I´m Still Not Dead
· Roxette The Look
· Slipknot Before I Forget
· Smash Mouth Walkin´ on the Sun
· The Smiths Stop Me If You´ve Heard This One Before
· The Sounds Living in America
· Spacehog In the Meantime
· Steve Miller Band Fly Like An Eagle
· Stone Temple Pilots Plush
· Swingin´ Utters This Bastard´s Life
· T.Rex 20th Century Boy
· Tears for Fears Everybody Wants to Rule the World
· Tegan and Sara The Con
· Them Crooked Vultures Dead End Friends
· Tokio Hotel Humanoid
· Tom Petty and The Heartbreakers I Need to Know
· The Vines Get Free
· War Low Rider
· Warren Zevon Werewolves of London
· The White Stripes The Hardest Button to Button
· Whitesnake Here I Go Again
· The Who I Can See for Miles
· Yes Roundabout